COMPARISON OF DIFFERENT EXTRACTION TECHNIQUES FOR ISOLATION OF TESTOSTERONE FROM SEA CUCUMBER (HOLOTHURIA SCABRA J)

Dewi, Kurnia Harlina and Masturah , Markom and Wan , Ramli Wan Daud and Devi , Silsia and Laili , Susanti (2016) COMPARISON OF DIFFERENT EXTRACTION TECHNIQUES FOR ISOLATION OF TESTOSTERONE FROM SEA CUCUMBER (HOLOTHURIA SCABRA J). In: 16th ASEAN Regional Symposium on Chemical Engineering, Philippines, Manila Hotel.

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Abstract

Testosterone, the steroid hormone, is not only produced by sea cucumber but a lot of other organisms as well, which is used to sex reversal and aphrodisiac. This research aimed to comparing the conventional extraction methods for isolation of testosterone from sea cucumber. The solvent is selected from previous study on reflux extraction with solvent of acetone, methanol, methanol- chloroform mixture (1:2) and chloroform solvent. Ratio of material: solvent are 1:1, 1:2 and 1:3 respectively. The highest result is obtained by successively use methanol: chloroform, acetone, chloroform and methanol mean equal to 0.2728 mg / 100g dry weight (dw), 0.2623 mg/100 g (dw), 0.1606 mg/100g (dw) and 0.0920 mg/100 g (dw). Influence of ratio showed increasing usage of solvent increase the result of extraction. The highest materials: solvent ratio is obtained at ratio 1:3, 1:2 and 1:1. The highest extraction in maceration extraction is obtained by using acetone at ratio 1:3 equal to 0.07721 mg/100 g dry weights

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