Variasi Genetik Mutan Anggrek Spathoglottis plicata Blume. Berdasarkan Marker ISSR

Romeida, Atra and Surjono, H. Sutjahjo and Agus, Purwito and Dewi, Sukma and Rustikawati , Rustikawati (2012) Variasi Genetik Mutan Anggrek Spathoglottis plicata Blume. Berdasarkan Marker ISSR. J. Agron. Indonesia, 40 (3). pp. 218-224. ISSN 2085-2916

[img]
Preview
Text (Journal)
6829-19209-1-PB.pdf - Published Version
Available under License Creative Commons GNU GPL (Software).

Download (388kB) | Preview

Abstract

Mutants of Spathoglottis plicata Blume were obtained from plants treated with Gamma Irradiation at 30-100 Gray. The mutants showed variations in the flower morphology. The objective of this experiment was to identify genetic variations of orchids S. plicata and its mutants using inter-simple sequent repeat (ISSR) markers. The amplified product of 10 ISSR primers produced 360 bands and 71 ISSR of the loci (90.14%) were polymorphic. The coefficient of similarity and principal component analysis produced five major groups with similarity coefficient of 0.68. The goodness of fit correlation matrix value reached 0.91. Therefore the ISSR isa good marker for identification of S. plicata mutants.

Item Type: Article
Subjects: S Agriculture > S Agriculture (General)
Divisions: Faculty of Agriculture > Department of Agroecotechnology
Depositing User: 001 Bambang Gonggo Murcitro
Date Deposited: 23 Oct 2013 11:44
Last Modified: 23 Oct 2013 11:44
URI: http://repository.unib.ac.id/id/eprint/1130

Actions (login required)

View Item View Item