DEVELOPMENT OF NEW TYPE UPLAND RICE LINES FOR RESISTANCE TO BLAST DISEASE THROUGH ANTHER CULTURE

Herawati, Reny and Bambang S. , Purwoko and Iswari S., Dewi (2016) DEVELOPMENT OF NEW TYPE UPLAND RICE LINES FOR RESISTANCE TO BLAST DISEASE THROUGH ANTHER CULTURE. In: Proceeding ISEPROLOCAL. Badan Penerbitan Fakultas Pertanian Universitas Bengkulu, Bengkulu, Indonesia, pp. 179-186. ISBN 9786029071184

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Abstract

The blast disease caused by Magnaporthe oryzae is an important disease on upland rice due to its rapid spreading rate andquite high to the host. One effort to overcome the obstacle is to use resistant varieties to the disease. Anther culture is one of tissue culture methods which can be applied to plant breeding programs in order to accelerate the process of obtaining pure lines. The successful development of rice varieties is highly dependent on genetic diversity and desirable traits. To obtain the genetic variability of doubled haploid lines through anther culture techniques, anther F1 or F2 were used as explants sources.The objective of the study were to develope of new plant type of upland rice through anther culture and to evaluate the resistance of the obtained double haploids(DH) to blast desease. Anthers from the F1plants of the crosses between Fatmawati and varieties of resistance to the bast disease were cultured and 348 DH lines were obtained. However, only 58 DH lines were suitable for upland rice development. After inoculating three isolates (race 173, 033, and 001) of M. oryzae, 26 DHlines showed resistance to all of the isolates. Lines P3-27, P5-50, P6-105, P3-162, and P3-204 possessed high yield potential with new plant type (more than six tillers), and resistance to blast desease.

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