IN-VITRO OVICIDAL AND LARVICIDAL ACTIVITY OF TWO LOCAL PLANTS (Ficus septicum AND Urena lobata) EXTRACTS ON Haemonchus contortus

Suteky, Tatik and Dwatmadji, Dwatmadji (2016) IN-VITRO OVICIDAL AND LARVICIDAL ACTIVITY OF TWO LOCAL PLANTS (Ficus septicum AND Urena lobata) EXTRACTS ON Haemonchus contortus. In: Proceeding ISEPROLOCAL. Badan Penerbitan Fakultas Pertanian Universitas Bengkulu, Bengkulu, Indonesia, pp. 295-299. ISBN 9786029071184

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Abstract

Haemonchus contortus is one of the health biggest constrains in a development of small ruminant production in all over the world. The annual cost for treatment is up to US $103 million a year, thus alternative method to control Haemonchus contortus created interest in research of the development of anthelmintic based on the local/medicinal plant. The purpose of this research is to examine the effect of crude aqueous extract of Ficus septicum and Urena lobata leaves on Haemonchus contortus eggs and larvae. Variables measure were ovicidal and larvicidal activity (egg hatch assay and larvae development assay) as well as adult motolity assay. The extracts were tested at 5 concentrations 3.125, 6.25, 12.5, 25, and 50 mg/ml. Oxfendazole or ivermectine super and distilled water were used as positive and negative control respectively. The crude aqueous extract of Urena lobata leaves showed highly significant (P<0.05) on ovicidal activity compared to the extract of Ficus septicum leaves. At concentration 50mg/ml extract inhibited larvae development by 65.40% (Ficus septicum) and 57.80% (Urena lobata). The two extracts showed reduced motility of worms about 40% two hours after incubation, while Oxbendazole inhibited motility of worm up to 80% two hours post exposure. In conclusion, Urena lobata leaves extract was more ovicidal than that of Ficus septicum leaves extracts. Further research on in-vivo experiment and toxicological evaluation are still needed.

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