FINGERPRINT STUDY OF MORINDAE CITRIFOLIAE FRUCTUS EXTRACTUM BY HIGH PERFORMANCE THIN LAYER CHROMATOGRAPHY (HPTLC)

Astuti, Sri and Herlina , B. Setijanti and Sri , Murhandini and Tepy , Usia (2016) FINGERPRINT STUDY OF MORINDAE CITRIFOLIAE FRUCTUS EXTRACTUM BY HIGH PERFORMANCE THIN LAYER CHROMATOGRAPHY (HPTLC). In: Proceeding ISEPROLOCAL. Badan Penerbitan Fakultas Pertanian Universitas Bengkulu, Bengkulu, Indonesia, pp. 394-397. ISBN 9786029071184

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Abstract

Research on fingerprint/chromatogram profile of Morindae citrifoliae Fructus (Noni fruit) extractum was done to develop a fingerprint of Noni fruit as one of the methods for quality control and standardization of herbal medicine. Samples were collected from three (3) areas: Bogor, Bandung, Yogyakarta, and its fingerprints were developed by HPTLC. The Stationary phase was HPTLC plates of silica gel 60 F254 and mobile phases was Ethyl acetate-methanol-water (100: 4: 4). TLC Photo Documentary System was used as detector at λ 366 nm and TLC Scanner at λ 343 nm to see the spectrum of scopoletin as a marker compound. Validation was done using specificity and precision parameters. The results showed that the scopoletin spectrum of the standard solution and test solution had appropriate acceptance criteria of specificity, as well as for precision and intermediate precision. In conclusion, the method was valid for the quality control and standardization of product based on the Noni fruit

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