Induced Mutation in Orchid (Spathoglottis plicata Blume) Originated from Bengkulu Province

Romeida, Atra and Surjono, H. Sutjahjo and A., Purwito and D., Sukma and Rustikawati, Rustikawati and S., Human (2014) Induced Mutation in Orchid (Spathoglottis plicata Blume) Originated from Bengkulu Province. In: International Seminar On Plant Mutation Breeding, Jakarta. (Unpublished)

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Abstract

Spathoglottis plicata Blume. is one of orchid species with lower level of geneticdiversity, especially in flower colorcompared tootherorchids. S. plicata accessions originated from Bengkulu usually producepink to purple flower with abright purple stems and flower stalk.Attemts to increase its genetic diversity had been done through induced mutation using Gammairradiation with doses of 30 to 100 Gy. The genetic variation resulted from induced mutation were then identified and analysed using morphological characters and ISSR markers.Morphological characterization was conducted by applying the Desriptor and Characterization list available at BALITHI, the Ministry of Agriculture. Molecular identification was done by using ten ISSR primers available at PKBT-IPB collection. Results of clusteranalysis in the second generation after irradiationtreatments produced five main groups of 12 genotypes at 0.68 coefficient of similarity with the goodness of fit correlation matrix value of 0.89 (classified as suitable). The amplified product of 10 ISSR primers produced 360 bands, where 71 ISSR of loci (91.11%) was polymorphic. Principal component analysis produced five major groups at 0.68 coefficient of similarity with the goodness of fit correlation matrix value 0.91 (classified as very suitable). It was concluded that genetic diversity existed among orchid mutants and this variation would be of important for further orchid breeding programs.

Item Type: Conference or Workshop Item (Poster)
Subjects: S Agriculture > S Agriculture (General)
Divisions: Faculty of Agriculture > Department of Agroecotechnology
Depositing User: 001 Bambang Gonggo Murcitro
Date Deposited: 14 Jun 2017 04:11
Last Modified: 14 Jun 2017 04:11
URI: http://repository.unib.ac.id/id/eprint/11745

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