SCREENING OF DOUBLE HAPLOID NEW PLANT TYPE UPLAND RICE FOR DROUGHT STRESS USING POLYETHYLENEGLYCOL (PEG) AND PROLINE ANALYSIS

Herawati, Reny and Purwoko, Bambang S and Iswari S., Dewi SCREENING OF DOUBLE HAPLOID NEW PLANT TYPE UPLAND RICE FOR DROUGHT STRESS USING POLYETHYLENEGLYCOL (PEG) AND PROLINE ANALYSIS. In: ISSAA 2017 International Symposium, March 28-29, 2017, Thailand,. (Unpublished)

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Abstract

The selection process of lines, especially to drought stress can be done by knowing the characteristics of physiology and biology as well as the morphology of the root system of each genotype. A number of doubled haploid (DH) lines were developed through anther culture from the cross of a new plant type (Fatmawati) and upland rice varieties. The purpose of this research was to identify doubled haploid lines tolerant to drought stress, as well as to determine the consistency of the polyethyleneglycol (PEG) 6000 test at germination stage and drought stress in the greenhouse. Materials used in this study were 78 double haploids (DH2) lines and 4 parents (SGJT-28, SGJT-36, Way Rarem, and Fatmawati), Jatiluhur and Cisokan as tolerance and sensitive control. The method used in this experiment were the early selection of doubled haploid (DH2) with 20% polyethyleneglycol (PEG) 6000 at germination stage and proline analysis at the vegetative stage. The results showed that PEG 6000 inhibited germination (33.9 percent), root length (60.8 percent) and shoot length (80 percent) of upland rice lines. Drought stress treatment (60 percent of field capacity) at the flowering period showed no significant reduction in the growth of doubled haploid upland rice but reduced the weight of grains per hill (52.11 percent). Drought stress decreased in total chlorophyll (20.7 μmol/cm) and increased proline content in leaves (30.3 μmol/g). Lines tested with polyethyleneglycol (PEG) 6000 were not all consistent with simulated drought stress in the greenhouse. Field experiment should be done to get accurate results.

Item Type: Conference or Workshop Item (Keynote)
Subjects: S Agriculture > SB Plant culture
Divisions: Faculty of Agriculture > Department of Agroecotechnology
Depositing User: Renny Herawati
Date Deposited: 28 Jul 2020 14:51
Last Modified: 28 Jul 2020 14:51
URI: http://repository.unib.ac.id/id/eprint/21355

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