PRELIMINARY TEST OF DETERMINATION OF ALCALOID AND STEROID COMPOUNDS AND BIOASSAY ON SOME VEGETABLE PLANT EXTRACT.

Ratnawati , Devi (2014) PRELIMINARY TEST OF DETERMINATION OF ALCALOID AND STEROID COMPOUNDS AND BIOASSAY ON SOME VEGETABLE PLANT EXTRACT. GRADIEN, 7 (2). pp. 692-696. ISSN 0216-2393

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Archive (Article) Gradien, Jurnal MIPA, ISSN 0216-2393, Vol. 7, No. 2, Juli 2011, hal 692-696.pdf - Bibliography Available under License Creative Commons GNU GPL (Software). Download (6MB) | Preview

Abstract

Preliminary test of determination of alkaloid and steroid compounds and biological activity on some vegetable plants had been carried out. The aim of this research was to test the presence of alkaloid and steroid compounds of vegetable leafs and to assay it’s biological activity.This research comprise two steps, the first step was to test presence of alkaloid and steroid compound and the second step was to investigates biological activity of alkaloid and steroid contained extracts plant using Brine Shrimp lethality test method. The result show that there were 9 species of 25 vegetable leafs containing alkaloids, one of them contain high alkaloids is papaya (carica papaya). Six species containing steroids, two of them contain high steroids are bayam leaf (amaranthus sp.) and melinjo leaf (gnetum gnemon).. In screen for biological activity (cytotoxic agents) of alkaloids or steroids plants, an extract of the leaves of bayam and melinjo as well as an extract of the pepaya leaf had LC50 < 1000 ppm, the value respectively are 153,1927 ppm, 48,2445 ppm, and 27,8090 ppm. Its means that three extract contain compounds'which have biological activity especially as cytotoxic.

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