INTROGRESSION OF CMV TOLERANCE GENES TO HYBRID PARENTS OF HOT PEPPER: EMPLOYING MORPHOLOGICAL AND RAPD MARKER TO IDENTIFY RECURRENT PARENT CHARACTERISTICS IN BC2 POPULATION

Herison , Catur and Winarsih, Sri and Handayaningsih , Merakati and Rustikawati , Rustikawati (2014) INTROGRESSION OF CMV TOLERANCE GENES TO HYBRID PARENTS OF HOT PEPPER: EMPLOYING MORPHOLOGICAL AND RAPD MARKER TO IDENTIFY RECURRENT PARENT CHARACTERISTICS IN BC2 POPULATION. In: International Seminar On Horticulture to Support Food Security 2010, 22-23 Juni 2010, Bandar Lampung.

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Abstract

. .superior and CMV,tolerant hybrid cultivarcan only be developed by crossing a pair of high heterobeltiosis parents and both of which are CMV tolerance parents. be accomplished if the tolerance is not exist in the parents. Gene introgression have to Tiris research was objlcted to emjtoy DNA markers on identiting backcrossing reccurent parent characteristics in BC2 cycle in CMV tolerance gene introgression. t"n"r"tio* to cut down the This re-searcn emptoyed five BC2 n!{u]1!9ns_generated from crosses of hybrid parenls PBC1354 and pBC378 with to'ierant parents of C1024, c1042 and C1043. The BC2 popuiations were selected ror their and were characterized^T o_ry. olo_gically_and 425, Ms of BC?A-([378t -ctr,tv tol"rrnce before molecularly. The results showed that genotype AZ4, l371l(378t1042'il-111);812, ai, sq of BC2B (t3tl4sT1i{sz.slcrbz+)]-61), C16, c33, C4 of Bc2c (t378tl37}l(3781c1d43)l-131) population were tolerance individuats and resemble to recurren-t hybrid parent PBC37B. Wnite'inOividuals D11, oss, ogg of BC2D (1-3!4{[13541(13s4tc_10a3)]-1BI); and individuals E1z, Ezo, E3i of BC2E ([1354/f 354lt13ulc1o24)141) population were CMV tolerance and resemble to recurrent hybrid parent P8c1354. Employing of both morphological and RAPD marker were efective to identify the recurent parent characteristics in early backcross generation.

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