UJI AKTIVITAS ANTIKANDIDIASIS EKSTRAK ETANOL 96% DAUN SENDUDUK BULU (Clidemia hirta ( L.) D.Don) TERHADAP KHAMIR Candida albicans

Candidiasis is an infection caused by the yeast C. albicans. C. albicans infection
often affects the vaginal area which is known as vulvovaginal candidiasis or
vaginal discharge. Candidiasis can be treated using traditional medicine or herbal
medicine derived from plants, one of which is the Senduduk Bulu plant (C. hirta).
This study aims to determine the phitochemical screening of 96% ethanol extract
of C. hirta leaves in inhibiting the growth of the yeast C. albicans, and to
determine the anti-candidiasis activity of 96% ethanol extract of C. hirta leaves in
inhibiting the growth of the yeast C. albicans at concentrations of 10%, 25%, 50%
and 75%. Activity test of 96% ethanol extract of C. hirta against yeast C. albicans
was analyzed by SPSS 25 application with ANOVA test analysis, from the results
obtained then continued with Duncan's test. Senduduk bulu leaf extract was made
by maceration method with 96% ethanol solvent. Phytochemical screening is seen
by the precipitation reaction and the color change reaction. The anticandidiasis
activity test was carried out with 6 treatments with 3 replications using the disc
diffusion method. The end result of making 96% ethanol extract of C.hirta is a
dark green viscous extract, with a yield calculation of 17.96%. Phytochemical test
results showed that C. hirta contained flavonoids, saponins, tannins and steroids.
C. hirta extract inhibited the growth of yeast C. albicans. The most effective
concentration of C. hirta extract in inhibiting the growth of the yeast C. albicans
was 75% with an inhibitory power of 28.73 mm, very strong category. The
ANOVA test results obtained a significance value of less than 0.05, which is
equal to 0.00 so that it has a significant effect. From ANOVA testing that has
been done, it can be concluded that 96% ethanol extract of C. hirta leaves has the
ability to inhibit the growt of C. albicans yeast.
Keywords : candidiasis, candida albicans, Clidemia hirta, infectious disease,
ethanol extraxt of senduduk bulu