IN VITRO PROPAGULE PRODUCTION OF SUPERIOR MANGOSTEEN (Garcinia mangostana L.)

Romeida, Atra and Wiryono, Wiryono (2016) IN VITRO PROPAGULE PRODUCTION OF SUPERIOR MANGOSTEEN (Garcinia mangostana L.). In: Proceeding ISEPROLOCAL. Badan Penerbitan Fakultas Pertanian Universitas Bengkulu, Bengkulu, Indonesia, pp. 94-97. ISBN 9786029071184

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Abstract

Mangosteen fruit is dubbed as the queen of tropical fruit. This experiment was aimed to produce mangosteen micro shoots in a large number rapidly using Benzyl Amino Purine (BAP) and Gibberellic acid (GA3). This study was conducted at the Agronomy laboratory, Agricultural Faculty, Bengkulu University from March to October 2013. This experiment used a completely randomized design (CRD) with 2 treatment factors. The first factor was BAP concentration, consisting of four levels, namely 0, 5, 10 and 15 mg L-1. The second factor was GA3 concentration, consisting of five levels, namely 0, 2.5, 5, 7.5, and 10 mg L-1. Every combination of treatment was repeated 5 times. One mangosteen seed sliced into four pieces was planted in each experimental unit and then planted at the Murashige and Skoog (MS) medium. The result showed that the highest number of micro shoots (99.33 shoots per explant) and the fastest formation of micro shoot (4 weeks after planting) were obtained at the 15 mg L-1 BAP and 10 mg L-1 GA3 combination.

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