PATHOGENICITY OF LOCAL ISOLATES ENTOMOPHATOGENIC FUNGI TO Diaphorina citri KUWAYAMA VECTOR OF CVPD DISEASE

Nadrawati, Nadrawati (2016) PATHOGENICITY OF LOCAL ISOLATES ENTOMOPHATOGENIC FUNGI TO Diaphorina citri KUWAYAMA VECTOR OF CVPD DISEASE. In: Proceeding ISEPROLOCAL. Badan Penerbitan Fakultas Pertanian Universitas Bengkulu, Bengkulu, Indonesia, pp. 452-456. ISBN 9786029071184

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Abstract

Diaphorina citri (Hemiptera: Psyllidae) is a major pest of citrus and CVPD disease vectors. Entomopathogenic fungi are one agent that can be used to control pests. The screening results have been found 3 Bengkulu local isolate potential for controlling D. citri. The purpose of this study was to determine the pathogenicity of entomopathogenic fungi isolates was indicated by the LC 50 and LC 80 on D. citri. The experimental design used was completely randomized design of 2 factors with 10 combinations, each repeated three times. The first factor is the type of entomopathogenic fungi and the second factor is the concentration of fungi conidia, that control (non-conidia), 1010 conidia/ml, 109 conidia/ml, 108 conidia/ml. The results showed that entomopathogenic fungi of Metarrhizium anisopliae and Beauveria bassiana were effective to D. citri nymph with 80-90% mortality on the eighth day after the application, the LC 50 and LC 80 M. anisopliae each others 0.1 x 107; 0.1 x 1010 conidia/ml, and B. bassiana 0.4 x 107; 0.8 x 1010 conidia/ml. Characterization of colony diameter and germination for all three isolates showed significantly different, the highest isolates of the colony diameter and germination found in Verticillium lecanii.

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