Plant Regeneration from Leaf Blade Explants of Horseradish (Amoracia rusticana L.) through In Vitro Culture

Yuliadi, E. (2008) Plant Regeneration from Leaf Blade Explants of Horseradish (Amoracia rusticana L.) through In Vitro Culture. Akta Agrosia, 11 (1). pp. 63-68. ISSN 1410-3354

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Abstract

Horseradish as an important crop in Japan is difficult to be propagated by using seed. The objective of this research was to overcome such difficulty through in vitro culture. The explants used were young leaves and old leaves. The MS basal medium was used and treated with 0; 0.1; 0.3; 0.6; 1.0 and 10 µM 2,4-D combinated with 0; 0.1; and 1.0 µM BA. This experiment showed that a combination of 0.1 µM 2,4-D and 0,.1 µM BA gave the highest plantlet regeneration as compared with 0; 1.0 and 10 µM 2,4-D combinated with 0 and 1.0 µM BA. Plantlet regeneration took place through direct morphogenesis. Sub-culturing calli from 1.0 µM 2,4-D and 0; 0.1 and 1.0 µM BA-supplemented medium to a medium without 2,4-D nor BA was the best sub-culturing method to get optimal plantlet regeneration. Young leaf explants had better response to plant growth regulators compared with old leaf explants.

Item Type: Article
Subjects: S Agriculture > S Agriculture (General)
Divisions: Faculty of Agriculture > Journal
Depositing User: Mr. Amwaluddin Lubis
Date Deposited: 23 Jun 2012 23:14
Last Modified: 23 Jun 2012 23:14
URI: http://repository.unib.ac.id/id/eprint/269

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