INDUCTION OF BACTERIAL WILT-FREE MICRORHIZOME OF GINGER IN A MODIFIED MS BASAL MEDIUM AND MACRONUTRIENT CONCENTRATION

Marlin, Marlin and Atra, Romeida and Hartal , Hartal and Bambang , Gonggo M (2013) INDUCTION OF BACTERIAL WILT-FREE MICRORHIZOME OF GINGER IN A MODIFIED MS BASAL MEDIUM AND MACRONUTRIENT CONCENTRATION. In: International Seminar on Spice, Medicinal, and Aromatic Plants. di Jakarta Convention Center, tanggal 29 Agustus 2013. Universitas Indonesia, Jakarta.

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Abstract

Healthy seedlings is a prerequisite to produce healthy plants. An experiment was conducted to determine an optimum concentration of macronutrient in MS basal medium, and a modified medium for stimulating microrhizome formation of big-white ginger Bacterial wilt-free ginger plantlets derived from 8 weeks cultured meristem in MS basal medium were cut into  5 mm length and used as the experimental material. Two-factor treatments were arranged in complete randomized design with three replications. The first factor was macronutrient concentration e.g. 25%, 50%, 75% of MS macronutrient, and full of MS macronutrient. The second factor was physic of medium e.g. soild medium, double layer, and double layer + CaP 1 ppm. Orthogonal polinomial tests showed that macronutrient concentration of 83.5% was the optimum concentration to produce microrhizome formation of ginger. In that microrhizome formation was the fastest (12.5 dap). On the otherhands , double layer medium with 25% of MS macronutrient promoted the highest number of shoot (5.5 shoots/explant) and root (33.4 roots/explant), and micro-rhizome dry weight (0,023 g). There was only 2% of sample showing R. solanacearum infection under microscopic observation. These implied that disease-free rhizome of ginger can be produced by in vitro propagation.

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