PERAKITAN GALUR PADI GOGO TOLERAN KEKERINGAN DAN TANAH BLAS BERDAYA HASIL TINGGI VARIETAS UNGGUL LOKAL BENGKULU MELALUI KULTUR ANTERA

Herawati, Reny and Rustikawati , Rustikawati and Entang , Inoriah Sukarjo (2010) PERAKITAN GALUR PADI GOGO TOLERAN KEKERINGAN DAN TANAH BLAS BERDAYA HASIL TINGGI VARIETAS UNGGUL LOKAL BENGKULU MELALUI KULTUR ANTERA. Project Report. Lembaga Penelitian Universitas Bengkulu. (Unpublished)

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Abstract

The use of improved varieties of upland rice is still very low, due to loack of avaibility of seed and lack of interest in producing rice seed breeder who excel. Difficulties increase rice production constraints caused by physical , biological and socio-economic. Cropping land generally reacted sourly with high Al saturation, but it often happens draoght and scarcity of food nutrients. Develope varieties require time and funds are relatively large. Estabilishment of homozygous lines can be accelerated with anther culture technique that can produce pure culture is very beneficial, because it does not need to be doubled haploid plants in rice anther selection. This study aims to determine the response of local varieties of indica rice crosses Bengkulu to callus induction and plants regenaration in anther culture. The main ingredient in this research is anther cross of rice plant (F1), which consists of local varieties with selected lines of P1 (Sriwijaya x IR-148), P2 (Sriwijaya x IR-7858-1), P3 (Bugis x IR-148), and P4 (Bugis x IR-7858-1), callus indoction media (N6), regeration medium (MS) (following the method of Dewi 2003; Herawati 2008). Anther culture of local varieties of rice indica/ japonica produced callus in induction and plant regeration are low, resulting in low efficiency of anther culture in providing green plants. The use of door parents indica/japonica which is responsive to anther culture into consideration for material F1 crosses. Anther inoculation needs to be done in several stage so that the opportunity to obtain plantlets in sufficient quantities for mmaterial selection will be greater.

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