IN VITRO PROPAGULE PRODUCTION OF SUPERIOR MANGOSTEEN (Garcinia mangostana L.)

Mangosteen fruit is dubbed as the queen of tropical fruit. This experiment was aimed to produce mangosteen
micro shoots in a large number rapidly using Benzyl Amino Purine (BAP) and Gibberellic acid (GA3). This
study was conducted at the Agronomy laboratory, Agricultural Faculty, Bengkulu University from March to
October 2013. This experiment used a completely randomized design (CRD) with 2 treatment factors. The first factor was BAP concentration, consisting of four levels, namely 0, 5, 10 and 15 mg L-1. The second factor was GA3 concentration, consisting of five levels, namely 0, 2.5, 5, 7.5, and 10 mg L-1. Every combination of
treatment was repeated 5 times. One mangosteen seed sliced into four pieces was planted in each experimental
unit and then planted at the Murashige and Skoog (MS) medium. The result showed that the highest number of
micro shoots (99.33 shoots per explant) and the fastest formation of micro shoot (4 weeks after planting) were
obtained at the 15 mg L-1 BAP and 10 mg L-1 GA3 combination.