FINGERPRINT STUDY OF Phyllanthus Niruri L. BY HIGH PERFORMANCE THIN LAYER CHROMATOGRAPHY (HPTLC)

Phyllanthus nirui L. (family Euphorbiaceae) has been used as traditional medicine for centuries. It has several
pharmacological properties for treating gastric lesion, urolithiasis, urinary infections and diuretics including
jaundice and anti-inflammatory property. To ensure the quality and prevent counterfeiting of the products, the
standardization of crude materials or extracts should be developed. Standardization can be done by comparing its
chromatogram profile or fingerprinting. The research on fingerprinting of P. niruri L. from various regions: Solo,
Bogor and Yogyakarta was conducted by High Performance Thin Layer Chromatography (HPTLC) using silica
gel F254 as a stationary phase and mix solution of chloroform : acetonitril : methanol : formic acid (60 : 30:10:
0.5) as a mobile phase. Preparation of the extracts was carried out in methanol. The extracts were eluted after
spoting 10 μL each using a Linomat-5 tools, then detected by a photo documentary system and TLC-Scanner on λ
254 nm, 229 nm and 366 nm. All extracts contained phyllanthin as a specific marker of P. niruri corresponding with reference standar, however the chromatogram profile of extract from Bogor had a bit different from Solo and Yogyakarta. In conclusion, the chromatogram/fingerprint profile can be used for standardization of P. niruri L extract as well as to verify the content of P. niruri L based product.