IN-VITRO OVICIDAL AND LARVICIDAL ACTIVITY OF TWO LOCAL PLANTS (Ficus septicum AND Urena lobata) EXTRACTS ON Haemonchus contortus

Haemonchus contortus is one of the health biggest constrains in a development of small ruminant production in
all over the world. The annual cost for treatment is up to US $103 million a year, thus alternative method to
control Haemonchus contortus created interest in research of the development of anthelmintic based on the
local/medicinal plant. The purpose of this research is to examine the effect of crude aqueous extract of Ficus
septicum and Urena lobata leaves on Haemonchus contortus eggs and larvae. Variables measure were ovicidal
and larvicidal activity (egg hatch assay and larvae development assay) as well as adult motolity assay. The
extracts were tested at 5 concentrations 3.125, 6.25, 12.5, 25, and 50 mg/ml. Oxfendazole or ivermectine super
and distilled water were used as positive and negative control respectively. The crude aqueous extract of Urena
lobata leaves showed highly significant (P<0.05) on ovicidal activity compared to the extract of Ficus septicum leaves. At concentration 50mg/ml extract inhibited larvae development by 65.40% (Ficus septicum) and 57.80% (Urena lobata). The two extracts showed reduced motility of worms about 40% two hours after incubation, while Oxbendazole inhibited motility of worm up to 80% two hours post exposure. In conclusion, Urena lobata leaves extract was more ovicidal than that of Ficus septicum leaves extracts. Further research on in-vivo experiment and toxicological evaluation are still needed.